Jennifer A. Gaddy, Ph.D.

Assistant Professor of Medicine


Division

Infectious Diseases

Education:

Ph.D. - Miami University, Oxford, Ohio

B.S. - Indiana University East, Richmond, Indiana

Dr. Gaddy’s research interests are focused on determining how bacteria interact with host cells and evade the immune system. In particular, her work has focused on mechanisms of metal homeostasis that alter bacterial virulence and determining how prokaryotic pathogens circumnavigate nutritional immunity within the vertebrate host. Dr. Gaddy’s research has revealed that nutrient iron and zinc availability affects the production and secretion of toxins by the gastric pathogen Helicobacter pylori. She employs numerous methods in her research including bacteriology, molecular biology, genetics, and biochemical, immunological, and high resolution imaging techniques. Dr. Gaddy was granted a Career Development Award (Level 2) from the Department of Veterans Affairs to pursue her research into the metal-dependent regulation of the Helicobacter pylori cag-Type IV secretion system that secretes the oncogenic cytotoxin, CagA.

Jennifer A. Gaddy, Ph.D.

publications 

Ackerman DL, Doster RS, Weitkamp JH, Aronoff DM, Gaddy JA, Townsend SD. Human Milk Oligosaccharides Exhibit Antimicrobial and Antibiofilm Properties against Group B StreptococcusACS Infect Dis [print-electronic]. 2017 Jun 6/13/2017; PMID: 28570820, DOI: 10.1021/acsinfecdis.7b00064, ISSN: 2373-8227. 

Jackson E, Little S, Franklin DS, Gaddy JA, Damo SM. Expression, Purification, and Antimicrobial Activity of S100A12J Vis Exp. 2017 May 5/13/2017; (123): PMID: 28570542, DOI: 10.3791/55557, ISSN: 1940-087X. 

Anders AP, Gaddy JA, Doster RS, Aronoff DM. Current concepts in maternal-fetal immunology: Recognition and response to microbial pathogens by decidual stromal cellsAm. J. Reprod. Immunol [print-electronic]. 2017 Mar; 77(3): PMID: 28044385, PMCID: PMC5321847, DOI: 10.1111/aji.12623, ISSN: 1600-0897. 

Doster RS, Kirk LA, Tetz LM, Rogers LM, Aronoff DM, Gaddy JAStaphylococcus aureus Infection of Human Gestational Membranes Induces Bacterial Biofilm Formation and Host Production of CytokinesJ. Infect. Dis. 2017 Feb 2/15/2017; 215(4): 653-7. PMID: 27436434, PII: jiw300, DOI: 10.1093/infdis/jiw300, ISSN: 1537-6613. 

Kothary V, Doster RS, Rogers LM, Kirk LA, Boyd KL, Romano-Keeler J, Haley KP, Manning SD, Aronoff DM, Gaddy JAGroup B Streptococcus Induces Neutrophil Recruitment to Gestational Tissues and Elaboration of Extracellular Traps and Nutritional ImmunityFront Cell Infect Microbiol. 2017; 7: 19. PMID: 28217556, PMCID: PMC5289994, DOI: 10.3389/fcimb.2017.00019, ISSN: 2235-2988. 

Gelbard A, Katsantonis NG, Mizuta M, Newcomb D, Rotsinger J, Rousseau B, Daniero JJ, Edell ES, Ekbom DC, Kasperbauer JL, Hillel AT, Yang L, Garrett CG, Netterville JL, Wootten CT, Francis DO, Stratton C, Jenkins K, McGregor TL, Gaddy JA, Blackwell TS, Drake WP. Molecular analysis of idiopathic subglottic stenosis for Mycobacterium speciesLaryngoscope [print-electronic]. 2017 Jan; 127(1): 179-85. PMID: 27295947, PMCID: PMC5156582, DOI: 10.1002/lary.26097, ISSN: 1531-4995. 

Johnson JG, Gaddy JA, DiRita VJ. The PAS Domain-Containing Protein HeuR Regulates Heme Uptake in Campylobacter jejuniMBio. 2016 Nov 11/15/2016; 7(6): PMID: 27935836, PMCID: PMC5111405, PII: mBio.01691-16, DOI: 10.1128/mBio.01691-16, ISSN: 2150-7511. 

Gelbard A, Katsantonis NG, Mizuta M, Newcomb D, Rotsinger J, Rousseau B, Daniero JJ, Edell ES, Ekbom DC, Kasperbauer JL, Hillel AT, Yang L, Garrett CG, Netterville JL, Wootten CT, Francis DO, Stratton C, Jenkins K, McGregor TL, Gaddy JA, Blackwell TS, Drake WP. Idiopathic subglottic stenosis is associated with activation of the inflammatory IL-17A/IL-23 axisLaryngoscope [print-electronic]. 2016 Nov; 126(11): E356-E361. PMID: 27296163, PMCID: PMC5077677, DOI: 10.1002/lary.26098, ISSN: 1531-4995. 

Servetas SL, Carpenter BM, Haley KP, Gilbreath JJ, Gaddy JA, Merrell DS. Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm FormationJ. Bacteriol [electronic-print]. 2016 Sep 9/15/2016; 198(18): 2536-48. PMID: 27432830, PMCID: PMC4999924, PII: JB.00324-16, DOI: 10.1128/JB.00324-16, ISSN: 1098-5530. 

Hurlow J, Blanz E, Gaddy JAClinical investigation of biofilm in non-healing wounds by high resolution microscopy techniquesJ Wound Care. 2016 Sep; 25 Suppl 9: S11-22. PMID: 27608736, PMCID: PMC5058422, DOI: 10.12968/jowc.2016.25.Sup9.S11, ISSN: 0969-0700. 

Wakeman CA, Moore JL, Noto MJ, Zhang Y, Singleton MD, Prentice BM, Gilston BA, Doster RS, Gaddy JA, Chazin WJ, Caprioli RM, Skaar EP. The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interactionNat Commun. 2016 Jun 6/15/2016; 7: 11951. PMID: 27301800, PMCID: PMC4912628, PII: ncomms11951, DOI: 10.1038/ncomms11951, ISSN: 2041-1723. 

Parker RE, Laut C, Gaddy JA, Zadoks RN, Davies HD, Manning SD. Association between genotypic diversity and biofilm production in group B StreptococcusBMC Microbiol. 2016 May 5/20/2016; 16: 86. PMID: 27206613, PMCID: PMC4875601, PII: 10.1186/s12866-016-0704-9, DOI: 10.1186/s12866-016-0704-9, ISSN: 1471-2180. 

Shaffer CL, Good JA, Kumar S, Krishnan KS, Gaddy JA, Loh JT, Chappell J, Almqvist F, Cover TL, Hadjifrangiskou M. Peptidomimetic Small Molecules Disrupt Type IV Secretion System Activity in Diverse Bacterial PathogensMBio. 2016 Apr 4/26/2016; 7(2): e00221-16. PMID: 27118587, PMCID: PMC4850256, PII: mBio.00221-16, DOI: 10.1128/mBio.00221-16, ISSN: 2150-7511. 

Haley KP, Gaddy JANutrition and Helicobacter pylori: Host Diet and Nutritional Immunity Influence Bacterial Virulence and Disease OutcomeGastroenterol Res Pract [print-electronic]. 2016; 2016: 3019362. PMID: 27688750, PMCID: PMC5027306, DOI: 10.1155/2016/3019362, ISSN: 1687-6121. 

Guevara C, Zhang C, Gaddy JA, Iqbal J, Guerra J, Greenberg DP, Decker MD, Carbonetti N, Starner TD, McCray PB, Mooi FR, Gómez-Duarte OG. Highly differentiated human airway epithelial cells: a model to study host cell-parasite interactions in pertussisInfect Dis (Lond) [print-electronic]. 2016; 48(3): 177-88. PMID: 26492208, PMCID: PMC5278880, DOI: 10.3109/23744235.2015.1100323, ISSN: 2374-4243. 

Gaddy JA, Radin JN, Cullen TW, Chazin WJ, Skaar EP, Trent MS, Algood HM. Helicobacter pylori Resists the Antimicrobial Activity of Calprotectin via Lipid A Modification and Associated Biofilm FormationMBio. 2015 Dec 12/8/2015; 6(6): e01349-15. PMID: 26646009, PMCID: PMC4669380, PII: mBio.01349-15, DOI: 10.1128/mBio.01349-15, ISSN: 2150-7511. 

Loh JT, Gaddy JA, Algood HM, Gaudieri S, Mallal S, Cover TL. Helicobacter pylori adaptation in vivo in response to a high-salt dietInfect. Immun [print-electronic]. 2015 Dec; 83(12): 4871-83. PMID: 26438795, PMCID: PMC4645402, PII: IAI.00918-15, DOI: 10.1128/IAI.00918-15, ISSN: 1098-5522. 

Haley KP, Delgado AG, Piazuelo MB, Mortensen BL, Correa P, Damo SM, Chazin WJ, Skaar EP, Gaddy JAThe Human Antimicrobial Protein Calgranulin C Participates in Control of Helicobacter pylori Growth and Regulation of VirulenceInfect. Immun [print-electronic]. 2015 Jul; 83(7): 2944-56. PMID: 25964473, PMCID: PMC4468547, PII: IAI.00544-15, DOI: 10.1128/IAI.00544-15, ISSN: 1098-5522. 

Noto JM, Lee JY, Gaddy JA, Cover TL, Amieva MR, Peek RM. Regulation of Helicobacter pylori Virulence Within the Context of Iron DeficiencyJ. Infect. Dis [print-electronic]. 2015 Jun 6/1/2015; 211(11): 1790-4. PMID: 25505301, PMCID: PMC4447831, PII: jiu805, DOI: 10.1093/infdis/jiu805, ISSN: 1537-6613. 

Haley KP, Gaddy JAMetalloregulation of Helicobacter pylori physiology and pathogenesisFront Microbiol. 2015; 6: 911. PMID: 26388855, PMCID: PMC4557348, DOI: 10.3389/fmicb.2015.00911, ISSN: 1664-302X. 

Haley KP, Gaddy JAHelicobacter pylori: Genomic Insight into the Host-Pathogen InteractionInt J Genomics [print-electronic]. 2015; 2015: 386905. PMID: 25722969, PMCID: PMC4334614, DOI: 10.1155/2015/386905, ISSN: 2314-436X. 

Haley KP, Blanz EJ, Gaddy JAHigh resolution electron microscopy of the Helicobacter pylori Cag type IV secretion system pili produced in varying conditions of iron availabilityJ Vis Exp. 2014 Nov 11/21/2014; (93): e52122. PMID: 25489938, PMCID: PMC4354248, DOI: 10.3791/52122, ISSN: 1940-087X. 

Gaddy JA, Radin JN, Loh JT, Piazuelo MB, Kehl-Fie TE, Delgado AG, Ilca FT, Peek RM, Cover TL, Chazin WJ, Skaar EP, Scott Algood HM. The host protein calprotectin modulates the Helicobacter pylori cag type IV secretion system via zinc sequestrationPLoS Pathog. 2014 Oct; 10(10): e1004450. PMID: 25330071, PMCID: PMC4199781, PII: PPATHOGENS-D-14-01130, DOI: 10.1371/journal.ppat.1004450, ISSN: 1553-7374. 

Granke M, Coulmier A, Uppuganti S, Gaddy JA, Does MD, Nyman JS. Insights into reference point indentation involving human cortical bone: sensitivity to tissue anisotropy and mechanical behaviorJ Mech Behav Biomed Mater [print-electronic]. 2014 Sep; 37: 174-85. PMID: 24929851, PMCID: PMC4112765, PII: S1751-6161(14)00141-6, DOI: 10.1016/j.jmbbm.2014.05.016, ISSN: 1878-0180. 

Johnson EM, Gaddy JA, Voss BJ, Hennig EE, Cover TL. Genes required for assembly of pili associated with the Helicobacter pylori cag type IV secretion systemInfect. Immun [print-electronic]. 2014 Aug; 82(8): 3457-70. PMID: 24891108, PMCID: PMC4136203, PII: IAI.01640-14, DOI: 10.1128/IAI.01640-14, ISSN: 1098-5522. 

Voss BJ, Gaddy JA, McDonald WH, Cover TL. Analysis of surface-exposed outer membrane proteins in Helicobacter pyloriJ. Bacteriol [print-electronic]. 2014 Jul; 196(13): 2455-71. PMID: 24769695, PMCID: PMC4054173, PII: JB.01768-14, DOI: 10.1128/JB.01768-14, ISSN: 1098-5530. 

Radin JN, González-Rivera C, Frick-Cheng AE, Sheng J, Gaddy JA, Rubin DH, Algood HM, McClain MS, Cover TL. Role of connexin 43 in Helicobacter pylori VacA-induced cell deathInfect. Immun [print-electronic]. 2014 Jan; 82(1): 423-32. PMID: 24191302, PMCID: PMC3911829, PII: IAI.00827-13, DOI: 10.1128/IAI.00827-13, ISSN: 1098-5522. 

McClain MS, Duncan SS, Gaddy JA, Cover TL. Control of gene expression in Helicobacter pylori using the Tet repressorJ. Microbiol. Methods [print-electronic]. 2013 Dec; 95(3): 336-41. PMID: 24113399, PMCID: PMC3856897, PII: S0167-7012(13)00302-3, DOI: 10.1016/j.mimet.2013.09.019, ISSN: 1872-8359. 

Gaddy JA, Radin JN, Loh JT, Zhang F, Washington MK, Peek RM, Algood HM, Cover TL. High dietary salt intake exacerbates Helicobacter pylori-induced gastric carcinogenesisInfect. Immun [print-electronic]. 2013 Jun; 81(6): 2258-67. PMID: 23569116, PMCID: PMC3676043, PII: IAI.01271-12, DOI: 10.1128/IAI.01271-12, ISSN: 1098-5522. 

Parekh VV, Wu L, Boyd KL, Williams JA, Gaddy JA, Olivares-Villagómez D, Cover TL, Zong WX, Zhang J, Van Kaer L. Impaired autophagy, defective T cell homeostasis, and a wasting syndrome in mice with a T cell-specific deletion of Vps34J. Immunol [print-electronic]. 2013 May 5/15/2013; 190(10): 5086-101. PMID: 23596309, PMCID: PMC3646937, PII: jimmunol.1202071, DOI: 10.4049/jimmunol.1202071, ISSN: 1550-6606. 

Radin JN, Gaddy JA, González-Rivera C, Loh JT, Algood HM, Cover TL. Flagellar localization of a Helicobacter pylori autotransporter proteinMBio. 2013 Apr 4/9/2013; 4(2): e00613-12. PMID: 23572556, PMCID: PMC3622936, PII: mBio.00613-12, DOI: 10.1128/mBio.00613-12, ISSN: 2150-7511. 

Noto JM, Gaddy JA, Lee JY, Piazuelo MB, Friedman DB, Colvin DC, Romero-Gallo J, Suarez G, Loh J, Slaughter JC, Tan S, Morgan DR, Wilson KT, Bravo LE, Correa P, Cover TL, Amieva MR, Peek RM. Iron deficiency accelerates Helicobacter pylori-induced carcinogenesis in rodents and humansJ. Clin. Invest [print-electronic]. 2013 Jan; 123(1): 479-92. PMID: 23257361, PMCID: PMC3533289, PII: 64373, DOI: 10.1172/JCI64373, ISSN: 1558-8238. 

Johnson EM, Gaddy JA, Cover TL. Alterations in Helicobacter pylori triggered by contact with gastric epithelial cellsFront Cell Infect Microbiol. 2012; 2: 17. PMID: 22919609, PMCID: PMC3417513, DOI: 10.3389/fcimb.2012.00017, ISSN: 2235-2988. 

Shaffer CL, Gaddy JA, Loh JT, Johnson EM, Hill S, Hennig EE, McClain MS, McDonald WH, Cover TL. Helicobacter pylori exploits a unique repertoire of type IV secretion system components for pilus assembly at the bacteria-host cell interfacePLoS Pathog [print-electronic]. 2011 Sep; 7(9): e1002237. PMID: 21909278, PMCID: PMC3164655, PII: PPATHOGENS-D-11-00533, DOI: 10.1371/journal.ppat.1002237, ISSN: 1553-7374.