Allison Galassie

Predoctoral Trainee, Department of Chemistry

Office Address:
1161 21st Avenue South, Medical Center North, Nashville, TN, TN 37232-2650

Allison Galassie

Professional Highlights 

Allison Galassie joined the Program on May 16, 2013.  Ms. Galassie is a chemistry PhD candidate working under the mentorship of Andrew Link, PhD, an expert on proteomics whose lab is studying proteomic “signatures” of blood cells involved in response to influenza virus vaccine. While supported by the IBVSTP, Allison is working on the analysis of the proteomics and immune response data from the 10-0074 clinical trial “A Randomized, Double-Blinded, Controlled, Phase I Study in Healthy Adults to Assess the Safety, Reactogenicity, and Immunogenicity of Intramuscular Subvirion Inactivated Monovalent Influenza A/H5N1 Virus Vaccine Administered With and Without AS03 Adjuvant: Standard & Systems Biology Analyses.”  In addition to this, she has completed related research projects while supported by the IBVSTP:

•     Sample proteomic prep for all human blood-derived samples of isolated B lymphocytes, T lymphocytes, Natural Killer (NK) cells, Monocytes, and Neutrophils

•     Completed quality control mass spectrometry runs of all proteomic samples

•     Completed mass spectrometry processing on the proteomic samples

•     Ran many samples for various collaborators as necessary

     Allison presented her work at the 62nd American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics in June, 2014 in Baltimore, MD.  Additionally, she presented an update on that same work at the 63rd ASMS Conference on Mass Spectrometry and Allied Topics in June, 2015 in St. Louis, MO. She is a co-author on a manuscript published in PLOS ONE that presents the experimental methods and computational approaches that were developed and optimized for the 10-0074 AS03-adjuvanted H5N1 Clinical Trial. Her review for the journal Proteomics - Clinical Applications that discusses the contributions of proteomics to vaccine research and development has been accepted for publication. Allison has successfully collected the quantitative proteomics data from the immune cells obtained from 20 subjects before and after vaccination in the 10-0074 AS03-H5N1 influenza clinical trial. She is writing a first-author manuscript that details her experimental findings and systems biology analysis. 

Research Description 

Recent Publications

Proteomic contributions to our understanding of vaccine and immune responses. Galassie AC, Link AJ. Proteomics Clin Appl. 2015 Dec;9(11-12):972-89. doi: 10.1002/prca.201500054. Epub 2015 Sep 10. Review. PMID: 26172619

A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccination. Hoek KL, Samir P, Howard LM, Niu X, Prasad N, Galassie A, Liu Q, Allos TM, Floyd KA, Guo Y, Shyr Y, Levy SE, Joyce S, Edwards KM, Link AJ. PLoS One. 2015 Feb 23;10(2):e0118528. doi: 10.1371/journal.pone.0118528. eCollection 2015. Erratum in: PLoS One. 2015;10(3):e0122550. PMID: 25706537