Predoctoral Trainee, Department of Pathology, Microbiology and Immunology
1161 21st Avenue South, Medical Center North, Nashville, TN, TN 37232-
Lindsay Nyhoff joined the training grant on June 01, 2013. Ms. Nyhoff is a graduate student in Microbiology and Immunology Program being mentored by Peggy Kendall, MD. Lindsay studies the mechanism of B lymphocyte signaling in autoimmune diseases. In her first year with the Program, she used phosphoflow cytometry to study anti-insulin anergic B cells in the context of Type 1 diabetes, and showed that these cells exhibited increased inhibitory signaling. These data supported the hypothesis that anergic B cells display low-level positive and negative signaling, resulting in increased dependence on positive signals during development. In the last year, Ms. Nyhoff’s embarked on another project based on Dr. Kendall’s previous observation that the genetic knockout of the Bruton’s Tyrosine Kinase (BTK) results in protection from T1D. This discovery has led Lindsey to study BTK role in another autoimmune disease, spontaneous autoimmune arthritis. Using spontaneous and serum-transfer models of arthritis, Ms. Nyhoff has found that BTK deficiency affords a significant protection from arthritis in a B cell-dependent mechanism. This work is reported in a new manuscript entitled “Bruton’s tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex mediated inflammatory arthritis,” which is in revision. Currently, Ms. Nyhoff and Dr. Kendall are developing the BTK-flox, tamoxifen-induced Cre system that will further allow them to explore the contribution of BTK signaling to autoimmune inflammation.
Lindsay Nyhoff was recognized at the 2014 Southeastern Immunology Symposium with an AAI Young Investigator award for Best Poster Abstract for “Antigen Specific Targeting of Autoreactive B Lymphocytes by siRNA Nanoparticles to Prevent T1D.” In addition, she co-authored another manuscript on "Bruton's Tyrosine Kinase Synergizes with Notch2 to Govern Marginal Zone B cells in Nonobese Diabetic Mice," published in Journal of Immunology. She is also a coauthor of the manuscript "Myeloid Dendritic Cells that Invade Islets of Langerhans Depend on S100A4 to Promote Autoimmune Diabetes" (in preparation).
Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex-mediated inflammatory arthritis. Nyhoff LE, Barron B, Johnson EM, Bonami RH, Maseda D, Fensterheim BA, Han W, Blackwell TS, Crofford LJ, Kendall PL. Arthritis Rheumatol. 2016 Mar 4. doi: 10.1002/art.39657. [Epub ahead of print] PMID: 26945549
The role of Bruton's tyrosine kinase in autoimmunity and implications for therapy. Crofford LJ, Nyhoff LE, Sheehan JH, Kendall PL. Expert Rev Clin Immunol. 2016 Mar 4:1-11. [Epub ahead of print] PMID: 26864273
Bruton's Tyrosine Kinase Synergizes with Notch2 To Govern Marginal Zone B Cells in Nonobese Diabetic Mice. Case JB, Bonami RH, Nyhoff LE, Steinberg HE, Sullivan AM, Kendall PL. J Immunol. 2015 Jul 1;195(1):61-70. doi: 10.4049/jimmunol.1400803. Epub 2015 Jun 1. PMID: 26034172